《植物生理学报》 2015, 51(12): 2195-2200

药用植物猴耳环的组织培养与快速繁殖

付姝颖¹, 潘超美^1,*, 刘良婷¹, 张家瑛¹, 苏家贤¹, 柳亚青¹, 杨森山², 邹国光²

¹广州中医药大学中药学院, 广州510006; ²广州莱泰制药有限公司, 广州510006

通信作者：潘超美；E-mail: pancm@gzucm.edu.cn；Tel: 020-39358249

摘　要：

以猴耳环成熟的种胚无菌萌发小苗为外植体, 研究不同种类及浓度的植物生长物质对丛芽诱导增殖和植株再生的影响, 建立完整的离体快繁体系。结果表明, 猴耳环种胚最佳灭菌方式为0.1% HgCl₂处理12 min, 污染率16.10%, 无菌萌发率78.66%; 丛芽初代诱导最佳培养基为MS+6-BA 0.5 mg•L^-1, 诱导率100%; 继代增殖最适宜的培养基为MS+6-BA 0.7 mg•L^-1+ NAA 0.05 mg•L^-1+IBA 0.3 mg•L^-1, 增殖系数3.95; 1/2MS+NAA 0.1 mg•L^-1+活性炭0.1 g•L^-1适合诱导生根, 45 d后可获得健壮植株, 生根率100%, 平均根数4.41。将生长良好的植株移栽到等体积比蛭石、沙子、泥炭土混合的基质中, 成活率66.67%。

关键词：猴耳环; 种胚; 组织培养; 快速繁殖

收稿：2015-09-21   修定：2015-10-26

资助：2014年国家中医药行业科研专项(201407002)。

Tissue Culture and Rapid Propagation of Medicinal Plant Pithecellobium clypearia

FU Shu-Ying¹, PAN Chao-Mei^1,*, LIU Liang-Ting¹, ZHANG Jia-Ying¹, SU Jia-Xian¹, LIU Ya-Qing¹, YANG Sen-Shan², ZOU Guo-Guang²

¹College of Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; ²Guangzhou LifeTech Pharmaceutical Co., Chinese Materia Media Ltd., Guangzhou 510006, China

Corresponding author: PAN Chao-Mei; E-mail: pancm@gzucm.edu.cn; Tel: 020-39358249

Abstract:

The seedlings sprouted from sterile Pithecellobium clypearia seed embryos were acted as explants. Effectiveness of different kinds and concentrations of plant growth regulators on the clusters producing and plant regeneration were compared to establish the rapid propagation technique by tissue culture. The results showed that the treatment with 0.1% HgCl2 for 12 min was suitable to sterilize P. clypearia seed embryos with the contamination rate of 16.10% and the sprout rate of 78.66%. The optimal medium for cluster buds induction was MS medium containing 0.5 mg•L^-1 6-BA, with the induction rate of 100%. The most suitable medium for cluster buds proliferation was MS+6-BA 0.7 mg•L^-1+NAA 0.05 mg•L^-1+IBA 0.3 mg•L^-1, and the proliferation coefficient reached 3.95. 1/2MS+NAA 0.1 mg•L^-1+AC 0.1 g•L^-1 was the optimum medium for plantlet rooting and the strong plant regeneration occurred 45 d later with the rooting rate up to 100% and the average number of roots up to 4.41. The plantlets were transplanted into the substratum composed of vermiculite, sand, and turf soil in equal volume proportion with a survival rate of 66.67%.

Key words: Pithecellobium clypearia; seed embryo; tissue culture; rapid propagation